Hotspot

rpl32-trnL-UAG

SSC · 110130-110712 · 583 bp

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Pi 0.0208
MarkerSeek score 56.10
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
583
variable_sites
9
pi
0.0208
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.74
markerseek_score
56.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AAAGCTTTTTCGTTAGCGCA GGTAAATGGCCCGAAAAAGC 40.0 50.0 57.2 58.0 898-1029 bp 1.0 78.7
rpl32-trnL-UAG_p2 AAGCTTTTTCGTTAGCGCAA GGTAAATGGCCCGAAAAAGC 40.0 50.0 57.2 58.0 897-1028 bp 1.0 78.7
rpl32-trnL-UAG_p3 AAAGCTTTTTCGTTAGCGCA TCCTAAGAGCAGCGTGTCTA 40.0 50.0 57.2 57.9 566-697 bp 1.0 78.1
rpl32-trnL-UAG_p4 AAGCTTTTTCGTTAGCGCAA TCCTAAGAGCAGCGTGTCTA 40.0 50.0 57.2 57.9 565-696 bp 1.0 78.1
rpl32-trnL-UAG_p5 AAGCTTTTTCGTTAGCGCAA AGCAGCGTGTCTACCAATTT 40.0 45.0 57.2 57.8 558-689 bp 1.0 77.7