Hotspot

pasI-ycf4

LSC · 58255-58414 · 160 bp

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Pi 0.0625
MarkerSeek score 75.30
Primer available yes
Label pasI-ycf4

Feature Metadata

label_name
pasI-ycf4
feature_type
igs
region
LSC
length_bp
160
variable_sites
10
pi
0.0625
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
75.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
pasI-ycf4_p1 TTTCCAGCAATTGCAATGGC TTTCGAGAAGGCCCTAGGAT 45.0 50.0 58.2 57.8 282-290 bp 1.0 79.9
pasI-ycf4_p2 CAGCAATTGCAATGGCTTCT TTTCGAGAAGGCCCTAGGAT 45.0 50.0 57.6 57.8 278-286 bp 1.0 78.4
pasI-ycf4_p3 TTTCCAGCAATTGCAATGGC AGAGCAAGGGTTTCGAGAAG 45.0 50.0 58.2 57.5 292-300 bp 1.0 77.5
pasI-ycf4_p4 TTTCCAGCAATTGCAATGGC GAGCAAGGGTTTCGAGAAGG 45.0 55.0 58.2 58.6 291-299 bp 1.0 76.7
pasI-ycf4_p5 CCAGCAATTGCAATGGCTTC TTTCGAGAAGGCCCTAGGAT 50.0 50.0 58.6 57.8 279-287 bp 1.0 76.3