Hotspot

atpF-atpA

LSC · 49698-49777 · 80 bp

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Pi 0.0625
MarkerSeek score 82.80
Primer available yes
Label atpF-atpA

Feature Metadata

label_name
atpF-atpA
feature_type
igs
region
LSC
length_bp
80
variable_sites
5
pi
0.0625
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
82.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpF-atpA_p1 TGTTTGGGGCGATGAAAGAA TGCTCAATGCGTTCACGTAT 45.0 45.0 58.0 58.3 165-166 bp 1.0 80.1
atpF-atpA_p2 ATTGGAATGTTTGGGGCGAT TGCTCAATGCGTTCACGTAT 45.0 45.0 58.1 58.3 172-173 bp 1.0 79.0
atpF-atpA_p3 AGCCTTACAGGGAGCTCTAG TGCTCAATGCGTTCACGTAT 55.0 45.0 57.9 58.3 248-249 bp 1.0 78.7
atpF-atpA_p4 ACAGGGAGCTCTAGGAACTC TGCTCAATGCGTTCACGTAT 55.0 45.0 57.8 58.3 242-243 bp 1.0 78.2
atpF-atpA_p5 TGTTTGGGGCGATGAAAGAA TCGCCTACTTGAAGTACGGT 45.0 50.0 58.0 58.5 219-220 bp 1.0 78.0