Hotspot

trnT-GGU-trnD-GUC

LSC · 29834-30116 · 283 bp

Back to Grona

Pi 0.0189
MarkerSeek score 63.80
Primer available yes
Label trnT-GGU-trnD-GUC

Feature Metadata

label_name
trnT-GGU-trnD-GUC
feature_type
igs
region
LSC
length_bp
283
variable_sites
5
pi
0.0189
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-trnD-GUC_p1 GAACCGATGACTTACGCCTT TTCAATTGGTCAGAGCACCG 50.0 50.0 58.0 58.5 393-402 bp 1.0 80.8
trnT-GGU-trnD-GUC_p2 CCATGGCGTTACTCTACCAC TTCAATTGGTCAGAGCACCG 55.0 50.0 58.1 58.5 372-381 bp 1.0 80.6
trnT-GGU-trnD-GUC_p3 TGGCGTTACTCTACCACTGA TTCAATTGGTCAGAGCACCG 50.0 50.0 58.1 58.5 369-378 bp 1.0 80.4
trnT-GGU-trnD-GUC_p4 CCGATGACTTACGCCTTACC TTCAATTGGTCAGAGCACCG 55.0 50.0 58.2 58.5 390-399 bp 1.0 79.6
trnT-GGU-trnD-GUC_p5 TTGAACCGATGACTTACGCC TTCAATTGGTCAGAGCACCG 50.0 50.0 58.3 58.5 395-404 bp 1.0 78.5