Hotspot

ycf3-psaA

LSC · 18699-19325 · 627 bp

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Pi 0.0112
MarkerSeek score 65.20
Primer available yes
Label ycf3-psaA

Feature Metadata

label_name
ycf3-psaA
feature_type
igs
region
LSC
length_bp
627
variable_sites
7
pi
0.0112
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-psaA_p1 AGCCCTTACCCCCAGTAATT ATGACCTGGTTTTGCCCATT 50.0 45.0 58.0 58.0 787 bp 1.0 80.5
ycf3-psaA_p2 AGCCCTTACCCCCAGTAATT AATGACCTGGTTTTGCCCAT 50.0 45.0 58.0 58.0 788 bp 1.0 80.4
ycf3-psaA_p3 AAGTTGCGGAGTCTTGGTTT AATGACCTGGTTTTGCCCAT 45.0 45.0 57.9 58.0 840 bp 1.0 80.0
ycf3-psaA_p4 AAGTTGCGGAGTCTTGGTTT ATGACCTGGTTTTGCCCATT 45.0 45.0 57.9 58.0 839 bp 1.0 80.0
ycf3-psaA_p5 AGCCCTTACCCCCAGTAATT TGCCCATTCCTCGAAAGAAG 50.0 50.0 58.0 57.9 775 bp 1.0 79.8