Hotspot

rbcL-atpB

LSC · 6655-7411 · 757 bp

Pi 0.00402

MarkerSeek score 61.40

Primer available yes

Label rbcL-atpB

Feature Metadata

label_name: rbcL-atpB
feature_type: igs
region: LSC
length_bp: 757
variable_sites: 3
pi: 0.00402
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-atpB_p1	AACACCAGCTTTGAACCCAA	CTACATCTAGTACCGGGCCA	45.0	55.0	58.1	58.0	905-908 bp	1.0	80.0
rbcL-atpB_p2	AACACCAGCTTTGAACCCAA	GTTTCAAGCGCAGAAACCTC	45.0	50.0	58.1	58.0	855-858 bp	1.0	79.8
rbcL-atpB_p3	AACACCAGCTTTGAACCCAA	CCTGGGGGAAAAGCTACATC	45.0	55.0	58.1	57.9	918-921 bp	1.0	79.5
rbcL-atpB_p4	AACACCAGCTTTGAACCCAA	ACATCTAGTACCGGGCCAAT	45.0	50.0	58.1	58.2	903-906 bp	1.0	78.4
rbcL-atpB_p5	GCTTTGAACCCAACACTTGC	CTACATCTAGTACCGGGCCA	50.0	55.0	58.4	58.0	898-901 bp	1.0	77.7