Hotspot

rpl36-infA

LSC · 82850-83118 · 269 bp

Back to Griffitharia

Pi 0.00186

MarkerSeek score 25.50

Primer available yes

Label rpl36-infA

Feature Metadata

label_name: rpl36-infA
feature_type: igs
region: LSC
length_bp: 269
variable_sites: 2
pi: 0.00186
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 25.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl36-infA_p1	TCGACCCCGTACATCTGTAA	AATCGGCGGAGAAGTTTTGT	50.0	45.0	57.9	58.1	914-964 bp	1.0	80.1
rpl36-infA_p2	ACCCCTTCTTGTACCCTTGA	AATCGGCGGAGAAGTTTTGT	50.0	45.0	57.9	58.1	968-1018 bp	1.0	80.0
rpl36-infA_p3	AGCTCTTAATGCCGCATCTC	AATCGGCGGAGAAGTTTTGT	50.0	45.0	58.1	58.1	1091-1141 bp	1.0	79.8
rpl36-infA_p4	GCTCTTAATGCCGCATCTCT	AATCGGCGGAGAAGTTTTGT	50.0	45.0	58.1	58.1	1090-1140 bp	1.0	79.8
rpl36-infA_p5	ACATCTGTACATCGGGTCCT	AATCGGCGGAGAAGTTTTGT	50.0	45.0	57.8	58.1	660 bp	1.0	78.5