Hotspot

ycf4-cemA

LSC · 63407-63963 · 557 bp

Back to Griffitharia

Pi 0.00269

MarkerSeek score 60.30

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 557
variable_sites: 4
pi: 0.00269
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TTGGCCTATTTCTTGCGTGT	AGAGAGATCCACCAAGGCAA	45.0	50.0	58.1	58.3	647-670 bp	1.0	79.8
ycf4-cemA_p2	AAAAAGCTGCTGAATTGGCC	AGAGAGATCCACCAAGGCAA	45.0	50.0	57.8	58.3	661-684 bp	1.0	79.1
ycf4-cemA_p3	AAAGCTGCTGAATTGGCCTA	AGAGAGATCCACCAAGGCAA	45.0	50.0	57.8	58.3	659-682 bp	1.0	78.7
ycf4-cemA_p4	TTGGCCTATTTCTTGCGTGT	GAGAGATCCACCAAGGCAAA	45.0	50.0	58.1	57.5	646-669 bp	1.0	78.5
ycf4-cemA_p5	TTGGCCTATTTCTTGCGTGT	AGAGAGAGATCCACCAAGGC	45.0	55.0	58.1	58.5	649-672 bp	1.0	78.4