Hotspot

accD-psaI

LSC · 61517-62327 · 811 bp

Back to Griffitharia

Pi 0.0044

MarkerSeek score 54.80

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 811
variable_sites: 11
pi: 0.0044
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AGGGCGTTCTGAGTGAGTTA	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.4	58.0	924-1021 bp	1.0	81.7
accD-psaI_p2	AGGGCGTTCTGAGTGAGTTA	AATGGAGGGGAGACTGTTGA	50.0	50.0	58.4	58.0	881-978 bp	1.0	81.6
accD-psaI_p3	AGGGCGTTCTGAGTGAGTTA	TTGCCGGAAATACTAAGCCC	50.0	50.0	58.4	58.0	915-1012 bp	1.0	81.6
accD-psaI_p4	AGGGCGTTCTGAGTGAGTTA	GAGGGGAGACTGTTGAGAGT	50.0	55.0	58.4	58.1	877-974 bp	1.0	81.5
accD-psaI_p5	AGGGCGTTCTGAGTGAGTTA	GCACAAAAATGGAGGGGAGA	50.0	50.0	58.4	58.1	888-985 bp	1.0	81.4