Hotspot

ndhC-trnV-UAC

LSC · 53607-54187 · 581 bp

Back to Griffitharia

Pi 0.00822

MarkerSeek score 63.60

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 581
variable_sites: 16
pi: 0.00822
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GGGGCTAAAACTCCGGAAAT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.9	57.8	700-748 bp	1.0	81.9
ndhC-trnV-UAC_p2	GGGGCTAAAACTCCGGAAAT	GCACGTTGGGTCTTTGAAAC	50.0	50.0	57.9	58.2	764-812 bp	1.0	81.8
ndhC-trnV-UAC_p3	GGGGCTAAAACTCCGGAAAT	ATGCACGTTGGGTCTTTGAA	50.0	45.0	57.9	58.3	766-814 bp	1.0	80.6
ndhC-trnV-UAC_p4	GGGGCTAAAACTCCGGAAAT	AATGCACGTTGGGTCTTTGA	50.0	45.0	57.9	58.3	767-815 bp	1.0	80.5
ndhC-trnV-UAC_p5	CTGGTCCTTTGCTAATCGGG	GCACGTTGGGTCTTTGAAAC	55.0	50.0	58.3	58.2	781-829 bp	1.0	80.1