Hotspot

rps4-trnT-UGU

LSC · 48450-48846 · 397 bp

Back to Griffitharia

Pi 0.0115

MarkerSeek score 55.00

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 397
variable_sites: 12
pi: 0.0115
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	TAAGATCGCTTCCGGCTCTA	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.0	57.9	540-585 bp	1.0	83.2
rps4-trnT-UGU_p2	TTTCTAAGATCGCTTCCGGC	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.1	57.9	544-589 bp	1.0	82.8
rps4-trnT-UGU_p3	TAAGATCGCTTCCGGCTCTA	ATGGTCATCGGTTCGATTCC	50.0	50.0	58.0	57.8	508-553 bp	1.0	82.6
rps4-trnT-UGU_p4	CTTCCGGCTCTAGGCTTTTT	CTCAGAGGTTAGAGCATCGC	50.0	55.0	57.9	57.9	532-577 bp	1.0	82.5
rps4-trnT-UGU_p5	TTTCTAAGATCGCTTCCGGC	ATGGTCATCGGTTCGATTCC	50.0	50.0	58.1	57.8	512-557 bp	1.0	82.2