Hotspot

trnT-GGU-psbD

LSC · 33700-35098 · 1399 bp

Back to Griffitharia

Pi 0.00288

MarkerSeek score 46.60

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1399
variable_sites: 15
pi: 0.00288
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	TCCCTCCGTAACCAGTCATC	55.0	55.0	58.1	58.5	1531-1562 bp	1.0	77.0
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	TCCCTCCGTAACCAGTCATC	50.0	55.0	58.1	58.5	1534-1565 bp	1.0	76.8
trnT-GGU-psbD_p3	GGCGTAAGTCATCGGTTCAA	TCCCTCCGTAACCAGTCATC	50.0	55.0	58.3	58.5	1508-1539 bp	1.0	75.3
trnT-GGU-psbD_p4	GTGGTAGAGTAACGCCATGG	CCTCCGTAACCAGTCATCCA	55.0	55.0	58.1	58.8	1529-1560 bp	1.0	74.7
trnT-GGU-psbD_p5	TCAGTGGTAGAGTAACGCCA	CCTCCGTAACCAGTCATCCA	50.0	55.0	58.1	58.8	1532-1563 bp	1.0	74.5