Hotspot

psbM-trnD-GUC

LSC · 31117-32368 · 1252 bp

Back to Griffitharia

Pi 0.00194

MarkerSeek score 55.30

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1252
variable_sites: 9
pi: 0.00194
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 55.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	TCACCTTGGCTGACTGTTTT	TTCAATTGGTCAGAGCACCG	45.0	50.0	57.6	58.5	1392-1441 bp	1.0	75.0
psbM-trnD-GUC_p2	AATCACCTTGGCTGACTGTT	TTCAATTGGTCAGAGCACCG	45.0	50.0	57.3	58.5	1394-1443 bp	1.0	73.2
psbM-trnD-GUC_p3	ATCACCTTGGCTGACTGTTT	TTCAATTGGTCAGAGCACCG	45.0	50.0	57.3	58.5	1393-1442 bp	1.0	73.2
psbM-trnD-GUC_p4	TCACCTTGGCTGACTGTTTT	GTTCAATTGGTCAGAGCACC	45.0	50.0	57.6	57.0	1393-1442 bp	1.0	70.9
psbM-trnD-GUC_p5	ATCACCTTGGCTGACTGTTTT	TTCAATTGGTCAGAGCACCG	42.9	50.0	58.1	58.5	1393-1442 bp	1.0	70.1