Hotspot

atpH-atpI

LSC · 14065-15259 · 1195 bp

Back to Griffitharia

Pi 0.00377

MarkerSeek score 62.90

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1195
variable_sites: 12
pi: 0.00377
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AATAGAAGCAAGCCCGACAG	GCGAATCCATGGAAGGTCAT	50.0	50.0	58.0	58.0	1278-1292 bp	1.0	81.0
atpH-atpI_p2	ATAACGGAAGCGGCAGAAAT	GCGAATCCATGGAAGGTCAT	45.0	50.0	58.0	58.0	1244-1258 bp	1.0	81.0
atpH-atpI_p3	AATAGAAGCAAGCCCGACAG	TTTTTGCAACCTTAGCTGCG	50.0	45.0	58.0	57.9	1308-1322 bp	1.0	80.2
atpH-atpI_p4	ATAACGGAAGCGGCAGAAAT	TTTTTGCAACCTTAGCTGCG	45.0	45.0	58.0	57.9	1274-1288 bp	1.0	80.1
atpH-atpI_p5	AATAACGGAAGCGGCAGAAA	GCGAATCCATGGAAGGTCAT	45.0	50.0	58.2	58.0	1245-1259 bp	1.0	79.8