Hotspot

trnR-UCU-atpA

LSC · 10113-10510 · 398 bp

Back to Griffitharia

Pi 0.0169

MarkerSeek score 61.70

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 398
variable_sites: 15
pi: 0.0169
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	TGGAATGAAAAGCGTCCATTG	AGACATTTACCGACGAAGCG	42.9	50.0	57.4	58.4	567-1241 bp	1.0	68.5
trnR-UCU-atpA_p2	GGAATGAAAAGCGTCCATTGT	AGACATTTACCGACGAAGCG	42.9	50.0	57.4	58.4	566-1240 bp	1.0	68.5
trnR-UCU-atpA_p3	GGTTCAAATCCTATTGGACGC	AGACATTTACCGACGAAGCG	47.6	50.0	57.3	58.4	506-1180 bp	1.0	67.9
trnR-UCU-atpA_p4	TGGAATGAAAAGCGTCCATT	AGACATTTACCGACGAAGCG	40.0	50.0	55.9	58.4	567-1241 bp	1.0	64.5
trnR-UCU-atpA_p5	TTGGAATGAAAAGCGTCCAT	AGACATTTACCGACGAAGCG	40.0	50.0	55.9	58.4	568-1242 bp	1.0	64.5