Hotspot

trnK-UUU-rps16

LSC · 4280-5236 · 957 bp

Back to Griffitharia

Pi 0.00557

MarkerSeek score 47.40

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 957
variable_sites: 13
pi: 0.00557
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 47.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	CCTTTCAGGATCAGTCGTGG	CGGAACTTCGCCCTAATCAA	55.0	50.0	58.0	58.0	1099-1139 bp	1.0	83.2
trnK-UUU-rps16_p2	TCAGGATCAGTCGTGGTCTT	CGGAACTTCGCCCTAATCAA	50.0	50.0	58.1	58.0	1095-1135 bp	1.0	82.8
trnK-UUU-rps16_p3	CCTTTCAGGATCAGTCGTGG	CGACGAGAGGGAAGAGATCT	55.0	55.0	58.0	58.0	1253-1293 bp	1.0	82.6
trnK-UUU-rps16_p4	AACCGTAGTAGGGCTCTACC	CGGAACTTCGCCCTAATCAA	55.0	50.0	57.9	58.0	1262-1302 bp	1.0	82.5
trnK-UUU-rps16_p5	CCTTTCAGGATCAGTCGTGG	GCCGTACGAGGAGAAAACTT	55.0	50.0	58.0	57.9	1357-1397 bp	1.0	82.1