Hotspot

rbcL

LSC · 57699-59141 · 1443 bp

Back to Grewia

Pi 0.00878
MarkerSeek score 52.10
Primer available yes
Label rbcL

Feature Metadata

label_name
rbcL
feature_type
gene
region
LSC
length_bp
1443
variable_sites
19
pi
0.00878
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL_p1 AGACCTTGTTGTTGCGAGAA ACAATGGGATCGGCTCAATC 45.0 50.0 57.7 58.0 1605-1608 bp 1.0 78.3
rbcL_p2 AGACCTTGTTGTTGCGAGAA GACAATGGGATCGGCTCAAT 45.0 50.0 57.7 58.0 1606-1609 bp 1.0 78.3
rbcL_p3 AGACCTTGTTGTTGCGAGAA GGGATCGGCTCAATCCTTTT 45.0 50.0 57.7 57.9 1600-1603 bp 1.0 78.0
rbcL_p4 AGACCTTGTTGTTGCGAGAA TGGGCCGAGTTTAATTGCAA 45.0 45.0 57.7 58.4 1570-1573 bp 1.0 75.5
rbcL_p5 AGACCTTGTTGTTGCGAGAA TTGGGCCGAGTTTAATTGCA 45.0 45.0 57.7 58.4 1571-1574 bp 1.0 75.5