Hotspot

psaC

SSC · 124444-124689 · 246 bp

Back to Grewia

Pi 0.0108
MarkerSeek score 37.70
Primer available yes
Label psaC

Feature Metadata

label_name
psaC
feature_type
gene
region
SSC
length_bp
246
variable_sites
4
pi
0.0108
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
37.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaC_p1 TGATGTTCAAAAATTTATAGATCCC CAGAAAAACCCGTGCTCAAA 28.0 45.0 53.9 57.1 386-388 bp 1.0 41.2
psaC_p2 TGATGTTCAAAAATTTATAGATCCC GTGGGGTTTTCTGGAACGTA 28.0 50.0 53.9 57.7 291 bp 1.0 41.1
psaC_p3 TGATGTTCAAAAATTTATAGATCCC AAGTGGGGTTTTCTGGAACG 28.0 50.0 53.9 58.3 293 bp 1.0 41.1
psaC_p4 ATGATGTTCAAAAATTTATAGATCCC GTGGGGTTTTCTGGAACGTA 26.9 50.0 54.4 57.7 292 bp 1.0 41.1
psaC_p5 ATGATGTTCAAAAATTTATAGATCCC AAGTGGGGTTTTCTGGAACG 26.9 50.0 54.4 58.3 294 bp 1.0 41.1