Hotspot

psaA

LSC · 41472-43724 · 2253 bp

Pi 0.00178

MarkerSeek score 58.30

Primer available yes

Label psaA

Feature Metadata

label_name: psaA
feature_type: gene
region: LSC
length_bp: 2253
variable_sites: 6
pi: 0.00178
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA_p1	GTCCTGAGCTAATCCTTGGC	GCGGGTCTCTTTGTATGTGT	55.0	50.0	58.0	57.9	2365 bp	1.0	79.1
psaA_p2	GTCCTGAGCTAATCCTTGGC	TTGGCGGGTCTCTTTGTATG	55.0	50.0	58.0	57.9	2368 bp	1.0	79.0
psaA_p3	GTCCTGAGCTAATCCTTGGC	ATGTGTTGTCCGGAAAGAGG	55.0	50.0	58.0	57.8	2351 bp	1.0	78.4
psaA_p4	AATACGACGAGTAGTGGGGT	GCGGGTCTCTTTGTATGTGT	50.0	50.0	57.9	57.9	2383 bp	1.0	78.4
psaA_p5	GTAGTGGGGTCCTGAGCTAA	GCGGGTCTCTTTGTATGTGT	55.0	50.0	58.1	57.9	2373 bp	1.0	78.3