Hotspot

rpl32-ndhF

SSC · 129191-130369 · 1179 bp

Back to Grewia

Pi 0.00396
MarkerSeek score 61.70
Primer available yes
Label rpl32-ndhF

Feature Metadata

label_name
rpl32-ndhF
feature_type
igs
region
SSC
length_bp
1179
variable_sites
7
pi
0.00396
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-ndhF_p1 TGAAAAAGCTTTCAACGCCG GGGAAGTGGCACAAAAGGTA 45.0 50.0 58.2 58.0 1329-1333 bp 1.0 79.3
rpl32-ndhF_p2 TGAAAAAGCTTTCAACGCCG AAATAGGAACGGGAAGTGGC 45.0 50.0 58.2 57.9 1339-1343 bp 1.0 78.4
rpl32-ndhF_p3 TGAAAAAGCTTTCAACGCCG TTTTGTTGCCGTCGGAAAAA 45.0 40.0 58.2 57.4 1380-1384 bp 1.0 74.2
rpl32-ndhF_p4 TGAAAAAGCTTTCAACGCCG TTTTTGTTGCCGTCGGAAAA 45.0 40.0 58.2 57.4 1381-1385 bp 1.0 74.2
rpl32-ndhF_p5 TTTCAACGCCGCCCAATAT GGGAAGTGGCACAAAAGGTA 47.4 50.0 58.1 58.0 1320-1324 bp 1.0 71.6