Hotspot

ndhD-ccsA

SSC · 126334-126645 · 312 bp

Back to Grewia

Pi 0.0107
MarkerSeek score 57.30
Primer available yes
Label ndhD-ccsA

Feature Metadata

label_name
ndhD-ccsA
feature_type
igs
region
SSC
length_bp
312
variable_sites
5
pi
0.0107
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhD-ccsA_p1 ATCAGGGGAGAAGGTCGAAA TGTGGCTTCTATGGGCTTTC 50.0 50.0 58.0 57.9 466-470 bp 1.0 79.6
ndhD-ccsA_p2 ACTATCAGGGGAGAAGGTCG TGTGGCTTCTATGGGCTTTC 55.0 50.0 57.9 57.9 469-473 bp 1.0 79.4
ndhD-ccsA_p3 ATCCGGATTTCGTCCTCTCA TGTGGCTTCTATGGGCTTTC 50.0 50.0 58.2 57.9 488-492 bp 1.0 78.2
ndhD-ccsA_p4 ATCAGGGGAGAAGGTCGAAA GTGGCTTCTATGGGCTTTCT 50.0 50.0 58.0 57.6 465-469 bp 1.0 77.2
ndhD-ccsA_p5 TCGTCCTCTCACTATCAGGG TGTGGCTTCTATGGGCTTTC 55.0 50.0 57.6 57.9 479-483 bp 1.0 77.2