Hotspot

trnT-UGU-trnL-UAA

LSC · 48887-49833 · 947 bp

Back to Grewia

Pi 0.00845
MarkerSeek score 51.70
Primer available yes
Label trnT-UGU-trnL-UAA

Feature Metadata

label_name
trnT-UGU-trnL-UAA
feature_type
igs
region
LSC
length_bp
947
variable_sites
12
pi
0.00845
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnL-UAA_p1 TAAGATCGCTTCCGGCTCTA TTCTCGCTTGCTGAACCTTT 50.0 45.0 58.0 58.0 1695-1711 bp 1.0 81.2
trnT-UGU-trnL-UAA_p2 TAAGATCGCTTCCGGCTCTA TAGCGTCTACCAATTTCGCC 50.0 50.0 58.0 58.1 1545-1561 bp 1.0 81.0
trnT-UGU-trnL-UAA_p3 TTTCTAAGATCGCTTCCGGC TTCTCGCTTGCTGAACCTTT 50.0 45.0 58.1 58.0 1699-1715 bp 1.0 80.9
trnT-UGU-trnL-UAA_p4 TAAGATCGCTTCCGGCTCTA ACCAATTTCGCCATATCCCC 50.0 50.0 58.0 58.0 1552-1553 bp 0.666667 67.3
trnT-UGU-trnL-UAA_p5 TTTCTAAGATCGCTTCCGGC ACCAATTTCGCCATATCCCC 50.0 50.0 58.1 58.0 1556-1557 bp 0.666667 66.9