Hotspot

psaA-ycf3

LSC · 43725-44525 · 801 bp

Back to Grewia

Pi 0.00832
MarkerSeek score 52.70
Primer available yes
Label psaA-ycf3

Feature Metadata

label_name
psaA-ycf3
feature_type
igs
region
LSC
length_bp
801
variable_sites
10
pi
0.00832
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaA-ycf3_p1 GCCCATTCCTCGAAAGAAGT TGGTTAAAGATCACGAGGCG 50.0 50.0 57.9 58.0 903-911 bp 1.0 80.6
psaA-ycf3_p2 GCCCATTCCTCGAAAGAAGT GGTTAAAGATCACGAGGCGT 50.0 50.0 57.9 58.0 902-910 bp 1.0 80.6
psaA-ycf3_p3 GCCCATTCCTCGAAAGAAGT GGAAACAGGCTATAGCGCTT 50.0 50.0 57.9 58.0 953-961 bp 1.0 80.2
psaA-ycf3_p4 TTCTTGAGAAATGACCCGGC TGGTTAAAGATCACGAGGCG 50.0 50.0 58.2 58.0 926-934 bp 1.0 80.1
psaA-ycf3_p5 TTCTTGAGAAATGACCCGGC GGTTAAAGATCACGAGGCGT 50.0 50.0 58.2 58.0 925-933 bp 1.0 80.1