Hotspot

rps2

LSC · 15644-16357 · 714 bp

Back to Gravesia

Pi 0

MarkerSeek score 40.00

Primer available yes

Label rps2

Feature Metadata

label_name: rps2
feature_type: gene
region: LSC
length_bp: 714
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2_p1	TCCCCACTCATTTTTATCTCA	TTCCGTCGGAACAATTGGTT	38.1	45.0	54.2	58.0	902 bp	1.0	41.5
rps2_p2	TCCCCACTCATTTTTATCTCA	CGTCCAATCCACGGTAGAAG	38.1	55.0	54.2	58.1	928 bp	1.0	41.1
rps2_p3	TCCCCACTCATTTTTATCTCA	TACGTCCAATCCACGGTAGA	38.1	50.0	54.2	57.9	930 bp	1.0	40.7
rps2_p4	TCCCCACTCATTTTTATCTCA	GAAGAAAAGGTTCCGTCGGA	38.1	50.0	54.2	57.8	912 bp	1.0	40.4
rps2_p5	TCCCCACTCATTTTTATCTCA	AGAAGAAAAGGTTCCGTCGG	38.1	50.0	54.2	57.8	913 bp	1.0	40.4