Hotspot

rps16

LSC · 4605-5659 · 1055 bp

Back to Gravesia

Pi 0

MarkerSeek score 40.00

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1055
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	AAAAAGAGGGAGGGAGAGGG	GTTAAAACCCGGGGGAATGT	55.0	50.0	58.3	58.1	1157 bp	1.0	76.8
rps16_p2	AAAAAGAGGGAGGGAGAGGG	AGTTAAAACCCGGGGGAATG	55.0	50.0	58.3	57.8	1158 bp	1.0	75.6
rps16_p3	AAAAAGAGGGAGGGAGAGGG	CAGTTAAAACCCGGGGGAAT	55.0	50.0	58.3	57.8	1159 bp	1.0	75.6
rps16_p4	AGGGAGAGGGGGATCTACTA	GTTAAAACCCGGGGGAATGT	55.0	50.0	57.4	58.1	1147 bp	1.0	75.0
rps16_p5	AGGGAGAGGGGGATCTACTA	AGTTAAAACCCGGGGGAATG	55.0	50.0	57.4	57.8	1148 bp	1.0	73.8