Hotspot

atpF

LSC · 11591-12962 · 1372 bp

Back to Gravesia

Pi 0

MarkerSeek score 40.00

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1372
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	CGTCGGCTTTAATGGTTACC	TGTAAAAGAAAGGGGATTGT	50.0	35.0	56.9	52.2	1466 bp	1.0	40.0
atpF_p2	CGTCGGCTTTAATGGTTACC	ATGTAAAAGAAAGGGGATTGT	50.0	33.3	56.9	52.9	1467 bp	1.0	40.0
atpF_p3	TCGTCGGCTTTAATGGTTACC	TGTAAAAGAAAGGGGATTGT	47.6	35.0	58.4	52.2	1467 bp	1.0	40.0
atpF_p4	TCGTCGGCTTTAATGGTTACC	ATGTAAAAGAAAGGGGATTGT	47.6	33.3	58.4	52.9	1468 bp	1.0	40.0
atpF_p5	CGTCGGCTTTAATGGTTACCA	TGTAAAAGAAAGGGGATTGT	47.6	35.0	58.7	52.2	1466 bp	1.0	40.0