Hotspot

trnC-GCA-petN

LSC · 28530-29382 · 853 bp

Back to Gravesia

Pi 0

MarkerSeek score 40.00

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 853
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	58.1	58.0	926 bp	1.0	79.3
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	CCTTTAGAGTCCACTTCGCC	50.0	55.0	58.1	58.0	985 bp	1.0	79.3
trnC-GCA-petN_p3	TTTCCCCAGTTCAAATCCGG	CTTTAGAGTCCACTTCGCCC	50.0	55.0	58.1	58.0	984 bp	1.0	79.3
trnC-GCA-petN_p4	TTTCCCCAGTTCAAATCCGG	CAGCCCAAGCAAGACTTACT	50.0	50.0	58.1	57.8	920 bp	1.0	77.8
trnC-GCA-petN_p5	TTTCCCCAGTTCAAATCCGG	AGACGACCATTAAAGCAGCC	50.0	50.0	58.1	58.3	935 bp	1.0	77.3