Hotspot

matK-trnK-UUU

LSC · 3452-4159 · 708 bp

Back to Gravesia

Pi 0

MarkerSeek score 40.00

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 708
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	AGGAAGTTGTGTTGCCAAGA	AACGGTAGAGTACTCGGCTT	45.0	50.0	57.6	58.2	788 bp	1.0	75.2
matK-trnK-UUU_p2	AGGAAGTTGTGTTGCCAAGA	ACGGTAGAGTACTCGGCTTT	45.0	50.0	57.6	58.2	787 bp	1.0	75.2
matK-trnK-UUU_p3	AGGAAGTTGTGTTGCCAAGA	GGGTTGCTAACTCAACGGTA	45.0	50.0	57.6	57.5	801 bp	1.0	72.8
matK-trnK-UUU_p4	AGGAAGTTGTGTTGCCAAGA	TTCGGGTTGCTAACTCAACG	45.0	50.0	57.6	58.5	804 bp	1.0	72.6
matK-trnK-UUU_p5	AGGAAGTTGTGTTGCCAAGAT	ACGGTAGAGTACTCGGCTTT	42.9	50.0	58.1	58.2	787 bp	1.0	70.2