Hotspot

rps5

Genome · 118832-119359 · 528 bp

Back to Grateloupia

Pi 0.0803

MarkerSeek score 79.50

Primer available yes

Label rps5

Feature Metadata

label_name: rps5
feature_type: gene
region: Genome
length_bp: 528
variable_sites: 61
pi: 0.0803
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 79.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps5_p1	GACTCCATTTTGCCCAAAGC	CAAGGTAGCGAGACTGTTCC	50.0	55.0	57.9	58.0	1099 bp	0.333333	52.6
rps5_p2	CGCCAACCCTATCTTTGTGA	CAAGGTAGCGAGACTGTTCC	50.0	55.0	57.9	58.0	933 bp	0.333333	52.5
rps5_p3	GACTCCATTTTGCCCAAAGC	AAGGTAGCGAGACTGTTCCT	50.0	50.0	57.9	58.1	1098 bp	0.333333	52.1
rps5_p4	CGCCAACCCTATCTTTGTGA	AAGGTAGCGAGACTGTTCCT	50.0	50.0	57.9	58.1	932 bp	0.333333	51.9
rps5_p5	GAAGCGCCATTACCTATCCC	CAAGGTAGCGAGACTGTTCC	55.0	55.0	58.2	58.0	1467 bp	0.333333	51.8