Hotspot

atpB

Genome · 154086-155507 · 1422 bp

Back to Grateloupia

Pi 0.0699

MarkerSeek score 79.40

Primer available yes

Label atpB

Feature Metadata

label_name: atpB
feature_type: gene
region: Genome
length_bp: 1422
variable_sites: 147
pi: 0.0699
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB_p1	GTTGCTTCAGCGAGTTCAAC	ATTATGCCGCGCTTACAGAA	50.0	45.0	58.0	58.1	2507 bp	0.333333	52.7
atpB_p2	GTTGTTGCTTCAGCGAGTTC	ATTATGCCGCGCTTACAGAA	50.0	45.0	58.0	58.1	2510 bp	0.333333	52.7
atpB_p3	TTGCTTCAGCGAGTTCAACT	ATTATGCCGCGCTTACAGAA	45.0	45.0	58.0	58.1	2506 bp	0.333333	52.5
atpB_p4	GTTGCTTCAGCGAGTTCAAC	TGTCAGCTCAATCAGAAGGC	50.0	50.0	58.0	57.9	2377 bp	0.333333	52.3
atpB_p5	GTTGTTGCTTCAGCGAGTTC	TGTCAGCTCAATCAGAAGGC	50.0	50.0	58.0	57.9	2380 bp	0.333333	52.3