Hotspot

clpC-rpl19

Genome · 100053-101083 · 1031 bp

Back to Grateloupia

Pi 0.214

MarkerSeek score 75.30

Primer available yes

Label clpC-rpl19

Feature Metadata

label_name: clpC-rpl19
feature_type: igs
region: Genome
length_bp: 1031
variable_sites: 225
pi: 0.214
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.70
markerseek_score: 75.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpC-rpl19_p1	CCCCAGTTGTCTTGCTTCTT	CCAAGAAGACGCTCTACCAC	50.0	55.0	58.0	58.0	2046 bp	0.333333	53.2
clpC-rpl19_p2	AGAAGGCCTACATCTAGCGT	CCAAGAAGACGCTCTACCAC	50.0	55.0	57.9	58.0	2495 bp	0.333333	52.7
clpC-rpl19_p3	CCCCAGTTGTCTTGCTTCTT	ACCGACCCATAAGGATGCTA	50.0	50.0	58.0	57.9	1927 bp	0.333333	52.5
clpC-rpl19_p4	CCCCAGTTGTCTTGCTTCTT	AGACGCTCTACCACTAAGCT	50.0	50.0	58.0	57.9	2040 bp	0.333333	52.2
clpC-rpl19_p5	CCCCAGTTGTCTTGCTTCTT	TTACTAATTGGGCCGGGTTG	50.0	50.0	58.0	57.9	2009 bp	0.333333	52.2