Hotspot

ompR-psbD

Genome · 69800-73273 · 3474 bp

Back to Grateloupia

Pi 0.21

MarkerSeek score 64.70

Primer available yes

Label ompR-psbD

Feature Metadata

label_name: ompR-psbD
feature_type: igs
region: Genome
length_bp: 3474
variable_sites: 308
pi: 0.21
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.30
markerseek_score: 64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ompR-psbD_p1	CTCAATAGGAAGAGGCTTGCA	TGCTTGCCATGAATACAGCT	47.6	45.0	57.7	57.9	1344 bp	0.333333	42.1
ompR-psbD_p2	CACTGTACCTATCATTCGCGA	TGCTTGCCATGAATACAGCT	47.6	45.0	57.7	57.9	813 bp	0.333333	41.5
ompR-psbD_p3	TCACTGTACCTATCATTCGCG	TGCTTGCCATGAATACAGCT	47.6	45.0	57.7	57.9	814 bp	0.333333	41.5
ompR-psbD_p4	CTCAATAGGAAGAGGCTTGCA	CCGCTCATCCGGTATTTCTC	47.6	55.0	57.7	58.2	1160 bp	0.333333	41.4
ompR-psbD_p5	CACTGTACCTATCATTCGCGA	CCGCTCATCCGGTATTTCTC	47.6	55.0	57.7	58.2	629 bp	0.333333	40.8