Hotspot

rrn16-ompR

Genome · 67365-69148 · 1784 bp

Back to Grateloupia

Pi 0.283

MarkerSeek score 79.40

Primer available yes

Label rrn16-ompR

Feature Metadata

label_name: rrn16-ompR
feature_type: igs
region: Genome
length_bp: 1784
variable_sites: 601
pi: 0.283
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn16-ompR_p1	CATTGCGGAAAATTCCCCAC	TGCTCCTAGTTCTAGTCCGG	50.0	55.0	58.0	57.9	2344 bp	0.333333	52.8
rrn16-ompR_p2	CATTGCGGAAAATTCCCCAC	GCTCCTAGTTCTAGTCCGGT	50.0	55.0	58.0	57.9	2343 bp	0.333333	52.8
rrn16-ompR_p3	AGCTACTGATCGTTGCCTTG	TGCTCCTAGTTCTAGTCCGG	50.0	55.0	58.0	57.9	2259 bp	0.333333	52.8
rrn16-ompR_p4	CAGCTACTGATCGTTGCCTT	TGCTCCTAGTTCTAGTCCGG	50.0	55.0	58.0	57.9	2260 bp	0.333333	52.8
rrn16-ompR_p5	AGCTACTGATCGTTGCCTTG	GCTCCTAGTTCTAGTCCGGT	50.0	55.0	58.0	57.9	2258 bp	0.333333	52.8