Hotspot

trnN-GUU-trnL-UAG

Genome · 43456-43627 · 172 bp

Back to Grateloupia

Pi 0.157
MarkerSeek score 80.50
Primer available yes
Label trnN-GUU-trnL-UAG

Feature Metadata

label_name
trnN-GUU-trnL-UAG
feature_type
igs
region
Genome
length_bp
172
variable_sites
36
pi
0.157
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.95
markerseek_score
80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GUU-trnL-UAG_p1 CTACCACTGAGCTACTGAGGA AGCGTGTCTACCAATTCCAC 52.4 50.0 58.0 57.9 213-222 bp 1.0 82.5
trnN-GUU-trnL-UAG_p2 TCTACCACTGAGCTACTGAGG AGCGTGTCTACCAATTCCAC 52.4 50.0 58.0 57.9 214-223 bp 1.0 82.5
trnN-GUU-trnL-UAG_p3 CTACCACTGAGCTACTGAGGA CTACCAATTCCACCACGTCC 52.4 55.0 58.0 58.3 206-215 bp 1.0 81.3
trnN-GUU-trnL-UAG_p4 TCTACCACTGAGCTACTGAGG CTACCAATTCCACCACGTCC 52.4 55.0 58.0 58.3 207-216 bp 1.0 81.3
trnN-GUU-trnL-UAG_p5 ACCAATCGGTTAACAGCCG AGCGTGTCTACCAATTCCAC 52.6 50.0 58.2 57.9 238-247 bp 1.0 81.0