Hotspot

ycf22-psaC

Genome · 16256-16980 · 725 bp

Back to Grateloupia

Pi 0.159

MarkerSeek score 83.40

Primer available yes

Label ycf22-psaC

Feature Metadata

label_name: ycf22-psaC
feature_type: igs
region: Genome
length_bp: 725
variable_sites: 156
pi: 0.159
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 83.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf22-psaC_p1	AAACGGGGATCATCAAGACG	CCCTCAGAGGATAAAACGGC	50.0	55.0	58.0	58.0	1561 bp	0.333333	52.8
ycf22-psaC_p2	AAACGGGGATCATCAAGACG	GGACGTAACATAGGTGCAGG	50.0	55.0	58.0	58.1	1801 bp	0.333333	52.6
ycf22-psaC_p3	AAACGGGGATCATCAAGACG	GGTCAAGCAGGACGTAACAT	50.0	50.0	58.0	57.9	1810 bp	0.333333	52.4
ycf22-psaC_p4	AAACGGGGATCATCAAGACG	TGGAACGGATGTAAAGCTGG	50.0	50.0	58.0	57.9	1219 bp	0.333333	52.3
ycf22-psaC_p5	AAACGGGGATCATCAAGACG	GGAACGGATGTAAAGCTGGT	50.0	50.0	58.0	57.9	1218 bp	0.333333	52.3