Hotspot

ycf1

SSC · 120471-125681 · 5211 bp

Back to Graptopetalum

Pi 0.00713

MarkerSeek score 57.00

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5211
variable_sites: 37
pi: 0.00713
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ACATCTAGCCAAAGAAATCTT	ATCGTTTACCTACCCACCCA	33.3	50.0	52.8	58.0	213-219 bp	1.0	44.4
ycf1_p2	CGCAATCTAGTTAATACATCTAGCC	ATCGTTTACCTACCCACCCA	40.0	50.0	57.5	58.0	228-234 bp	1.0	44.2
ycf1_p3	CGCAATCTAGTTAATACATCTAGCC	TCGTTTACCTACCCACCCAG	40.0	55.0	57.5	58.7	227-233 bp	1.0	44.2
ycf1_p4	ACGCAATCTAGTTAATACATCTAGC	ATCGTTTACCTACCCACCCA	36.0	50.0	56.9	58.0	229-235 bp	1.0	44.1
ycf1_p5	TCTTTTTGATCAATACGCAATCT	ATCGTTTACCTACCCACCCA	30.4	50.0	55.0	58.0	243-249 bp	1.0	43.9