Hotspot

ndhD

SSC · 112629-114131 · 1503 bp

Back to Graptopetalum

Pi 0.00399

MarkerSeek score 47.90

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 6
pi: 0.00399
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	TAGTTGCAGTCGTCAAGAGC	ACGATGTGAATCTGCCTGTC	50.0	50.0	57.9	58.0	1914-1916 bp	1.0	80.6
ndhD_p2	TAGGGATTCTTTCCGGAGCA	ACGATGTGAATCTGCCTGTC	50.0	50.0	58.1	58.0	2239-2241 bp	1.0	80.1
ndhD_p3	AATGAGGCCTGGGGATCATA	ACGATGTGAATCTGCCTGTC	50.0	50.0	57.9	58.0	2210-2212 bp	1.0	80.0
ndhD_p4	TGAGGCCTGGGGATCATATT	ACGATGTGAATCTGCCTGTC	50.0	50.0	57.9	58.0	2208-2210 bp	1.0	80.0
ndhD_p5	AAGCACCTCGTTTGATTCGT	ACGATGTGAATCTGCCTGTC	45.0	50.0	58.1	58.0	2346 bp	0.5	58.9