Hotspot

trnN-GUU-trnR-ACG

IRa · 126077-126668 · 592 bp

Back to Graptopetalum

Pi 0.00507

MarkerSeek score 61.50

Primer available yes

Label trnN-GUU-trnR-ACG

Feature Metadata

label_name: trnN-GUU-trnR-ACG
feature_type: igs
region: IRa
length_bp: 592
variable_sites: 3
pi: 0.00507
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-trnR-ACG_p1	TGGTCGTAGGTTCGAATCCT	ACAGATCTAGTGGAGACGGG	50.0	55.0	58.2	57.9	720 bp	1.0	78.6
trnN-GUU-trnR-ACG_p2	TGGTCGTAGGTTCGAATCCT	CTCCCGGGAACAGATCTAGT	50.0	55.0	58.2	57.9	729 bp	1.0	78.5
trnN-GUU-trnR-ACG_p3	CGAATCTCTCGTTGGTCCTC	ACAGATCTAGTGGAGACGGG	55.0	55.0	57.8	57.9	777 bp	1.0	78.3
trnN-GUU-trnR-ACG_p4	CGAATCTCTCGTTGGTCCTC	CTCCCGGGAACAGATCTAGT	55.0	55.0	57.8	57.9	786 bp	1.0	78.1
trnN-GUU-trnR-ACG_p5	ATCTCTCGTTGGTCCTCAGT	ACAGATCTAGTGGAGACGGG	50.0	55.0	57.8	57.9	774 bp	1.0	78.0