Hotspot

trnR-ACG-trnN-GUU

IRb · 105707-106298 · 592 bp

Back to Graptopetalum

Pi 0.00507
MarkerSeek score 61.50
Primer available yes
Label trnR-ACG-trnN-GUU

Feature Metadata

label_name
trnR-ACG-trnN-GUU
feature_type
igs
region
IRb
length_bp
592
variable_sites
3
pi
0.00507
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-ACG-trnN-GUU_p1 ACAGATCTAGTGGAGACGGG TGGTCGTAGGTTCGAATCCT 55.0 50.0 57.9 58.2 720 bp 1.0 78.6
trnR-ACG-trnN-GUU_p2 CTCCCGGGAACAGATCTAGT TGGTCGTAGGTTCGAATCCT 55.0 50.0 57.9 58.2 729 bp 1.0 78.5
trnR-ACG-trnN-GUU_p3 ACAGATCTAGTGGAGACGGG CGAATCTCTCGTTGGTCCTC 55.0 55.0 57.9 57.8 777 bp 1.0 78.3
trnR-ACG-trnN-GUU_p4 CTCCCGGGAACAGATCTAGT CGAATCTCTCGTTGGTCCTC 55.0 55.0 57.9 57.8 786 bp 1.0 78.1
trnR-ACG-trnN-GUU_p5 ACAGATCTAGTGGAGACGGG ATCTCTCGTTGGTCCTCAGT 55.0 50.0 57.9 57.8 774 bp 1.0 78.0