Hotspot

rpl20-rps12

LSC · 66457-67562 · 1106 bp

Back to Graptopetalum

Pi 0.00706

MarkerSeek score 62.30

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 1106
variable_sites: 6
pi: 0.00706
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.77
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	CTGAGTCGACTGCTCAAACA	CCTGGAAGCGGAAGAACTAC	50.0	55.0	57.9	58.0	1857-2180 bp	1.0	83.2
rpl20-rps12_p2	ACTGAGTCGACTGCTCAAAC	CCTGGAAGCGGAAGAACTAC	50.0	55.0	57.9	58.0	1858-2181 bp	1.0	83.2
rpl20-rps12_p3	CTGAGTCGACTGCTCAAACA	AGCTCACGGAATTGTGGATC	50.0	50.0	57.9	58.0	1725-2048 bp	1.0	83.1
rpl20-rps12_p4	CTGAGTCGACTGCTCAAACA	GCTCACGGAATTGTGGATCT	50.0	50.0	57.9	58.0	1724-2047 bp	1.0	83.1
rpl20-rps12_p5	CTGAGTCGACTGCTCAAACA	ATCCATCAACCTGCGAGTTC	50.0	50.0	57.9	58.0	1907-2230 bp	1.0	83.0