Hotspot

psbE-petL

LSC · 62417-63501 · 1085 bp

Back to Graptopetalum

Pi 0.00281

MarkerSeek score 62.00

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1085
variable_sites: 3
pi: 0.00281
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	CGTATCTTGCTCAGCCCAAT	50.0	50.0	57.9	58.0	1258-1275 bp	1.0	80.6
psbE-petL_p2	AGAACGTTCTCCTGTGCTTC	CGTATCTTGCTCAGCCCAAT	50.0	50.0	57.8	58.0	1181-1198 bp	1.0	79.8
psbE-petL_p3	ACGAATAACCAACCCGCAAT	CGTATCTTGCTCAGCCCAAT	45.0	50.0	58.2	58.0	1267-1284 bp	1.0	79.6
psbE-petL_p4	CGAATAACCAACCCGCAATG	CGTATCTTGCTCAGCCCAAT	50.0	50.0	57.8	58.0	1266-1283 bp	1.0	79.5
psbE-petL_p5	GTTCTCCTGTGCTTCCAGAC	CGTATCTTGCTCAGCCCAAT	55.0	50.0	58.2	58.0	1176-1193 bp	1.0	79.5