Hotspot

trnD-GUC-trnY-GUA

LSC · 30375-30686 · 312 bp

Back to Graptopetalum

Pi 0.0161

MarkerSeek score 62.30

Primer available yes

Label trnD-GUC-trnY-GUA

Feature Metadata

label_name: trnD-GUC-trnY-GUA
feature_type: igs
region: LSC
length_bp: 312
variable_sites: 5
pi: 0.0161
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-trnY-GUA_p1	ATATTGGAGACGTCGGGACT	AAGTGATTGTTCCTGGGTCG	50.0	50.0	57.9	57.8	496-497 bp	1.0	78.9
trnD-GUC-trnY-GUA_p2	ATATTGGAGACGTCGGGACT	TGGCAATATGTCTACGCTGG	50.0	50.0	57.9	57.8	438-439 bp	1.0	78.6
trnD-GUC-trnY-GUA_p3	ATATTGGAGACGTCGGGACT	GGCAATATGTCTACGCTGGT	50.0	50.0	57.9	57.8	437-438 bp	1.0	78.6
trnD-GUC-trnY-GUA_p4	ATATTGGAGACGTCGGGACT	GGGGACGGACTGTAAATTCG	50.0	55.0	57.9	58.4	459-460 bp	1.0	77.7
trnD-GUC-trnY-GUA_p5	ATATTGGAGACGTCGGGACT	GGGACGGACTGTAAATTCGT	50.0	50.0	57.9	57.6	458-459 bp	1.0	77.5