Hotspot

trnS-GCU-trnG-GCC

LSC · 8580-9208 · 629 bp

Back to Graptopetalum

Pi 0.0159

MarkerSeek score 65.30

Primer available yes

Label trnS-GCU-trnG-GCC

Feature Metadata

label_name: trnS-GCU-trnG-GCC
feature_type: igs
region: LSC
length_bp: 629
variable_sites: 10
pi: 0.0159
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-GCC_p1	CAATCCGACGCTTTAGTCCA	TGTTCGGAATACAAAGAAAACCG	50.0	39.1	58.0	57.9	753-764 bp	1.0	56.2
trnS-GCU-trnG-GCC_p2	AATCCGACGCTTTAGTCCAC	TGTTCGGAATACAAAGAAAACCG	50.0	39.1	58.0	57.9	752-763 bp	1.0	56.2
trnS-GCU-trnG-GCC_p3	GCTCGTACAACGGATTAGCA	TGTTCGGAATACAAAGAAAACCG	50.0	39.1	58.1	57.9	771-782 bp	1.0	55.6
trnS-GCU-trnG-GCC_p4	CAATCCGACGCTTTAGTCCA	CGAAGGACCCCTTAACTATTAGG	50.0	47.8	58.0	57.8	732-743 bp	1.0	55.4
trnS-GCU-trnG-GCC_p5	AATCCGACGCTTTAGTCCAC	CGAAGGACCCCTTAACTATTAGG	50.0	47.8	58.0	57.8	731-742 bp	1.0	55.3