Hotspot

ycf38

Genome · 144514-145410 · 897 bp

Back to Gracilariopsis

Pi 0.0721

MarkerSeek score 80.10

Primer available yes

Label ycf38

Feature Metadata

label_name: ycf38
feature_type: gene
region: Genome
length_bp: 897
variable_sites: 94
pi: 0.0721
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf38_p1	CAACCTCTTCAGCTGCATCT	TGGAAAGCACCGAATCCAAA	50.0	45.0	57.9	58.0	1819 bp	0.333333	52.4
ycf38_p2	CAACCTCTTCAGCTGCATCT	AGAGCTGGTTCTCCTGTTCT	50.0	50.0	57.9	58.0	1747 bp	0.333333	52.3
ycf38_p3	CAACCTCTTCAGCTGCATCT	TGTTACCCCTAGTCTTGCCA	50.0	50.0	57.9	58.0	1565 bp	0.333333	52.2
ycf38_p4	ACCTTGTCCTATCTGCTCGT	TGGAAAGCACCGAATCCAAA	50.0	45.0	58.1	58.0	1941 bp	0.333333	52.1
ycf38_p5	CAACCTCTTCAGCTGCATCT	TACTCCAACCTCCCCAAGAG	50.0	55.0	57.9	58.0	1587 bp	0.333333	52.1