Hotspot

ycf23

Genome · 50816-51616 · 801 bp

Back to Gracilariopsis

Pi 0.108

MarkerSeek score 82.30

Primer available yes

Label ycf23

Feature Metadata

label_name: ycf23
feature_type: gene
region: Genome
length_bp: 801
variable_sites: 123
pi: 0.108
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf23_p1	GGTTGTAAGCGCTGTGAAAC	GCTGAGAAATTCTTGTAGCAGC	50.0	45.5	58.0	58.0	1334-1602 bp	0.666667	62.3
ycf23_p2	AGGTTGTAAGCGCTGTGAAA	GCTGAGAAATTCTTGTAGCAGC	45.0	45.5	58.0	58.0	1335-1603 bp	0.666667	62.3
ycf23_p3	GGTTGTAAGCGCTGTGAAAC	CGTCAAAACGCTGAGAAATTCT	50.0	40.9	58.0	57.9	1611 bp	0.333333	36.0
ycf23_p4	AGGTTGTAAGCGCTGTGAAA	CGTCAAAACGCTGAGAAATTCT	45.0	40.9	58.0	57.9	1612 bp	0.333333	36.0
ycf23_p5	CTCAATGTGTACGTGCATGC	GCTGAGAAATTCTTGTAGCAGC	50.0	45.5	57.8	58.0	1706 bp	0.333333	35.9