Hotspot

thiG

Genome · 6645-7475 · 831 bp

Back to Gracilariopsis

Pi 0.0715

MarkerSeek score 80.30

Primer available yes

Label thiG

Feature Metadata

label_name: thiG
feature_type: gene
region: Genome
length_bp: 831
variable_sites: 87
pi: 0.0715
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
thiG_p1	AAGCACGAAAAGTTGCTCCT	AACGAACAGCAGGAAAAGGA	45.0	45.0	58.3	57.7	1854-1858 bp	0.666667	63.6
thiG_p2	AACCTTATGAATGGGCGGTC	AACGAACAGCAGGAAAAGGA	50.0	45.0	57.9	57.7	1793 bp	0.333333	50.2
thiG_p3	TATGAATGGGCGGTCAGGTA	AACGAACAGCAGGAAAAGGA	50.0	45.0	58.2	57.7	1788 bp	0.333333	49.0
thiG_p4	TGAATGGGCGGTCAGGTATA	AACGAACAGCAGGAAAAGGA	50.0	45.0	58.2	57.7	1786 bp	0.333333	49.0
thiG_p5	AACCTTATGAATGGGCGGTC	ACGAACAGCAGGAAAAGGAT	50.0	45.0	57.9	57.4	1792 bp	0.333333	48.4