Hotspot

orf445

Genome · 73620-74957 · 1338 bp

Back to Gracilariopsis

Pi 0.151

MarkerSeek score 78.80

Primer available yes

Label orf445

Feature Metadata

label_name: orf445
feature_type: gene
region: Genome
length_bp: 1338
variable_sites: 296
pi: 0.151
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
orf445_p1	TCAAGGCTATCAATGGACTAACA	CCATCACGAAACCCTAACAGA	39.1	47.6	57.3	57.7	2068 bp	0.333333	13.3
orf445_p2	TCAAGGCTATCAATGGACTAACA	TCCATCACGAAACCCTAACAG	39.1	47.6	57.3	57.7	2069 bp	0.333333	13.3
orf445_p3	TCAAGGCTATCAATGGACTAACA	ATGATCCATCACGAAACCCT	39.1	45.0	57.3	56.6	2073 bp	0.333333	13.3
orf445_p4	TCAAGGCTATCAATGGACTAACA	TCCATCACGAAACCCTAACA	39.1	45.0	57.3	56.5	2069 bp	0.333333	13.3
orf445_p5	TCAAGGCTATCAATGGACTAACA	AATGATCCATCACGAAACCCT	39.1	42.9	57.3	57.3	2074 bp	0.333333	13.3