Hotspot

rrn5-rps6

Genome · 4896-5535 · 640 bp

Back to Gracilariopsis

Pi 0.187

MarkerSeek score 75.10

Primer available yes

Label rrn5-rps6

Feature Metadata

label_name: rrn5-rps6
feature_type: igs
region: Genome
length_bp: 640
variable_sites: 103
pi: 0.187
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.70
markerseek_score: 75.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn5-rps6_p1	ATCTCCCCCAAGAGTTCACA	GACCGCCCATTCATAAGGTT	50.0	50.0	58.0	57.9	1841 bp	0.333333	52.6
rrn5-rps6_p2	TCTCCCCCAAGAGTTCACAT	GACCGCCCATTCATAAGGTT	50.0	50.0	58.0	57.9	1840 bp	0.333333	52.6
rrn5-rps6_p3	CAAGTCGAGCAGAGACGAAA	GACCGCCCATTCATAAGGTT	50.0	50.0	57.9	57.9	1941 bp	0.333333	52.5
rrn5-rps6_p4	TTCGGTCCATATCCGGTGTA	GACCGCCCATTCATAAGGTT	50.0	50.0	57.9	57.9	1696 bp	0.333333	52.3
rrn5-rps6_p5	CCTTAGTGATCCGACAGTGC	GACCGCCCATTCATAAGGTT	55.0	50.0	58.1	57.9	1916 bp	0.333333	52.3