Hotspot

rns-trnI(gat)

Genome · 1481-1583 · 103 bp

Back to Gracilariopsis

Pi 0.129

MarkerSeek score 82.90

Primer available yes

Label rns-trnI(gat)

Feature Metadata

label_name: rns-trnI(gat)
feature_type: igs
region: Genome
length_bp: 103
variable_sites: 19
pi: 0.129
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rns-trnI(gat)_p1	GGGGTGAAGTCGTAACAAGG	GGTGGGCTATCCTGGATTTG	55.0	55.0	58.2	58.0	227-236 bp	1.0	88.3
rns-trnI(gat)_p2	CAAGGTAGCCGTACTGGAAG	GGTGGGCTATCCTGGATTTG	55.0	55.0	57.7	58.0	212-221 bp	1.0	88.2
rns-trnI(gat)_p3	GGGGTGAAGTCGTAACAAGG	GGATTTGAACCAGGGACCTC	55.0	55.0	58.2	57.9	214-223 bp	1.0	87.9
rns-trnI(gat)_p4	AGTAACTGGGGTGAAGTCGT	GGTGGGCTATCCTGGATTTG	50.0	55.0	58.3	58.0	234-243 bp	1.0	87.3
rns-trnI(gat)_p5	GGGGTGAAGTCGTAACAAGG	ATGGTGGGCTATCCTGGATT	55.0	50.0	58.2	58.2	229-238 bp	1.0	87.0