Hotspot

ycf37

Genome · 26895-27365 · 471 bp

Back to Gracilaria

Pi 0.188

MarkerSeek score 82.00

Primer available yes

Label ycf37

Feature Metadata

label_name: ycf37
feature_type: gene
region: Genome
length_bp: 471
variable_sites: 271
pi: 0.188
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf37_p1	GTTCAAGTCCTGCTATCCCG	CGAGCCCTAAAGCCAGATTT	55.0	50.0	58.1	58.0	689-738 bp	0.823529	84.1
ycf37_p2	GTTCAAGTCCTGCTATCCCG	TAGACTCATGCGAGCCCTAA	55.0	50.0	58.1	57.9	699-748 bp	0.294118	62.8
ycf37_p3	GTTCAAGTCCTGCTATCCCG	CTGGATAGACTCATGCGAGC	55.0	55.0	58.1	57.9	704-753 bp	0.176471	58.1
ycf37_p4	GCTAAACGGGATATAGCGCA	CGAGCCCTAAAGCCAGATTT	50.0	50.0	57.9	58.0	748-797 bp	0.117647	51.7
ycf37_p5	TGCTAAACGGGATATAGCGC	CGAGCCCTAAAGCCAGATTT	50.0	50.0	57.9	58.0	749-798 bp	0.117647	51.7